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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
28/12/2023 |
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Data da última atualização: |
22/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROSA, P. M. da S.; GUEDES, P. H. E.; GARCIA, J. M.; OLIVEIRA, C. S. |
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Afiliação: |
PAOLA MARIA DA SILVA ROSA, UNIVERSIDADE ESTADUAL PAULISTA; PEDRO HENRIQUE EVAGELISTA GUEDES; JOAQUIM MANSANO GARCIA, UNIVERSIDADE ESTADUAL PAULISTA; CLARA SLADE OLIVEIRA, CNPGL. |
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Título: |
Cytoplasmic granules in bovine oocytes do not affect embryonic or fetal development. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Zygote, v. 32, n. 1, p. 28-37, 2023. |
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DOI: |
https://doi.org/10.1017/S0967199423000576 |
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Idioma: |
Inglês |
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Conteúdo: |
Oocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus–oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspase 3 levels (66.17 ± 11.61 vs 172.08 ± 16.95, P < 0.01) and similar GAP junction activity (5.64 ± 0.45 vs 6.29 ± 0.29). ZAR1 relative mRNA amount was lower in granular oocytes (178.27 ± 151.63 vs 0.89 ± 0.89, P = 0.01) and no effect was detected for MATER, PPP2R1A, ENY2, IGF2R, and BMP15 genes. Despite molecular differences, no detrimental effect was detected on oocyte competence in GC oocytes. Cleavage (Experiment 1: 59.52 ± 7.21% vs 59.79 ± 6.10% and Experiment 2: 68.88 ± 4.82 vs 74.41 ± 5.89%) and blastocyst (Experiment 1: 29.28 ± 4.14% vs 23.15 ± 2.96% and Experiment 2: 21.11 ± 3.28% vs 21.02 ± 6.08%) rates were similar between CC and GC (Experiments 1 and 2, respectively). Post-transfer embryo development revealed that pregnancy (CC: 24.27 ± 9.70% vs GC: 26.31 ± 7.23%) and calving (23.68% vs 33.33%) rates and fetal growth were not affected by the presence of cytoplasmic granules. Our results demonstrated that oocytes with granular cytoplasm present equivalent efficiency for IVF and calf production compared with homogenous cytoplasm oocytes. This could be observed through similar cleavage, blastocyst rates, and fetal growth development. In addition to differences in oocyte gene expression related to oocyte quality, it seems not to affect oocyte developmental competence. MenosOocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus–oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspase 3 levels (66.17 ± 11.61 vs 172.08 ± 16.95, P < 0.01) and similar GAP junction activity (5.64 ± 0.45 vs 6.29 ± 0.29). ZAR1 relative mRNA amount was lower in granular oocytes (178.27 ± 151.63 vs 0.89 ± 0.89, P = 0.01) and no effect was detected for MATER, PPP2R1A, ENY2, IGF2R, and BMP15 genes. Despite molecular differences, no detrimental effect was detected on oocyte competence in GC oocytes. Cleavage (Experiment 1: 59.52 ± 7.21% vs 59.79 ± 6.10% and Experiment 2: 68.88 ± 4.82 vs 74.41 ± 5.89%) and blastocyst (Experiment 1: 29.28 ± 4.14% vs 23.15 ± 2.96% and Experiment 2: 21.11 ± 3.28% vs 21.02 ± 6.08%) rates were similar between CC and GC (Experiments 1 and 2, respectively). Post-transfer embryo development revealed that pregnancy (CC: 24.27 ± 9.70% vs GC: 26.31 ± 7.23%) and calving (23.68% vs 33.33%) rates and fetal growth were not affected by the presence of cytoplasmic granules. Our results demonstrated that oocytes with granular cytopla... Mostrar Tudo |
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Palavras-Chave: |
Classificação morfológica; IVF; Oócito; Ooplasma; Ovócito. |
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Thesagro: |
Bovino; Citoplasma; Embrião Animal; Granulo. |
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Thesaurus Nal: |
Cytoplasm; Granules. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02681naa a2200301 a 4500
001 2160283
005 2024-01-22
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S0967199423000576$2DOI
100 1 $aROSA, P. M. da S.
245 $aCytoplasmic granules in bovine oocytes do not affect embryonic or fetal development.$h[electronic resource]
260 $c2023
520 $aOocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus–oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspase 3 levels (66.17 ± 11.61 vs 172.08 ± 16.95, P < 0.01) and similar GAP junction activity (5.64 ± 0.45 vs 6.29 ± 0.29). ZAR1 relative mRNA amount was lower in granular oocytes (178.27 ± 151.63 vs 0.89 ± 0.89, P = 0.01) and no effect was detected for MATER, PPP2R1A, ENY2, IGF2R, and BMP15 genes. Despite molecular differences, no detrimental effect was detected on oocyte competence in GC oocytes. Cleavage (Experiment 1: 59.52 ± 7.21% vs 59.79 ± 6.10% and Experiment 2: 68.88 ± 4.82 vs 74.41 ± 5.89%) and blastocyst (Experiment 1: 29.28 ± 4.14% vs 23.15 ± 2.96% and Experiment 2: 21.11 ± 3.28% vs 21.02 ± 6.08%) rates were similar between CC and GC (Experiments 1 and 2, respectively). Post-transfer embryo development revealed that pregnancy (CC: 24.27 ± 9.70% vs GC: 26.31 ± 7.23%) and calving (23.68% vs 33.33%) rates and fetal growth were not affected by the presence of cytoplasmic granules. Our results demonstrated that oocytes with granular cytoplasm present equivalent efficiency for IVF and calf production compared with homogenous cytoplasm oocytes. This could be observed through similar cleavage, blastocyst rates, and fetal growth development. In addition to differences in oocyte gene expression related to oocyte quality, it seems not to affect oocyte developmental competence.
650 $aCytoplasm
650 $aGranules
650 $aBovino
650 $aCitoplasma
650 $aEmbrião Animal
650 $aGranulo
653 $aClassificação morfológica
653 $aIVF
653 $aOócito
653 $aOoplasma
653 $aOvócito
700 1 $aGUEDES, P. H. E.
700 1 $aGARCIA, J. M.
700 1 $aOLIVEIRA, C. S.
773 $tZygote$gv. 32, n. 1, p. 28-37, 2023.
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Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
22/01/2015 |
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Data da última atualização: |
22/01/2015 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
ASSIS, L. A. G. de; HANADA, R. E.; COELHO NETTO, R. A.; GASPAROTTO, L. |
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Afiliação: |
LUADIR GASPAROTTO, CPAA. |
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Título: |
Doenças do jatobá, oiti, pau-rosa e pau-de-balsa. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: GASPAROTTO, L.; BENTES, J. L. da S.; PEREIRA, J. C. R. (Ed.). Doenças de espécies florestais arbóreas nativas e exóticas na Amazônia. Brasília, DF: Embrapa, 2014. p. 45-61. |
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Idioma: |
Português |
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Palavras-Chave: |
Oiti; Pau-de-balsa; Pau-rosa. |
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Thesagro: |
Doença de Planta; Jatobá. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00675naa a2200205 a 4500
001 2006406
005 2015-01-22
008 2014 bl uuuu u00u1 u #d
100 1 $aASSIS, L. A. G. de
245 $aDoenças do jatobá, oiti, pau-rosa e pau-de-balsa.
260 $c2014
650 $aDoença de Planta
650 $aJatobá
653 $aOiti
653 $aPau-de-balsa
653 $aPau-rosa
700 1 $aHANADA, R. E.
700 1 $aCOELHO NETTO, R. A.
700 1 $aGASPAROTTO, L.
773 $tIn: GASPAROTTO, L.; BENTES, J. L. da S.; PEREIRA, J. C. R. (Ed.). Doenças de espécies florestais arbóreas nativas e exóticas na Amazônia. Brasília, DF: Embrapa, 2014. p. 45-61.
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